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单词 EcoRV
释义

EcoRV

中文百科

EcoRV

EcoRV与DNA形成的复合体。
EcoRV cleaving DNA. The protein loosely binds DNA and scans for its recognition sequence. Once found, EcoRV kinks the DNA in a 50° angle and cleaves at the cognate sequence.

EcoRV(V是5的意思)是一种第二型限制内切酶,来自某些特定品系的大肠杆菌(Escherichia coli),此细菌的学名也是EcoRV前三个字母的由来。

EcoRV是分子生物学与生物技术上常使用的限制酶,可辨识出一段含有6个碱基的5'GAT|ATC-3'(|为切开的位置)DNA串行,以及互补的3'-CTA|TAG-5',以产生平整末端(blunt end)的方式将此串行切开。

英语百科

EcoRV

EcoRV cleaving DNA. The protein loosely binds DNA and scans for its recognition sequence. Once found, EcoRV kinks the DNA in a 50° angle and cleaves at the cognate sequence.

EcoRV (pronounced "eco R five") is a type II restriction endonuclease isolated from certain strains of Escherichia coli. It has the alternative name Eco32I.

In molecular biology, it is a commonly used restriction enzyme. It creates blunt ends. The enzyme recognizes the palindromic 6-base DNA sequence 5'-GAT|ATC-3' and makes a cut at the vertical line. The complementary sequence is then 3'-CTA|TAG-5'. The ends are blunt and can be ligated into a blunt cloning site easily but with lower efficiency than sticky ends.

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更新时间:2025/6/27 9:19:14