Immunogold labelling

Immunogold labeling or Immunogold staining (IGS) is a staining technique used in electron microscopy. This staining technique follow the same patterns of the Indirect immunofluorescence : colloidal gold particles are most often attached to secondary antibodies which are in turn attached to primary antibodies designed to bind a specific antigen or other cell component. Gold is used for its high electron density which increases electron scatter to give high contrast 'dark spots'.