丙酮酸羧化酶，属于连接酶类的酶，催化（根据不同的物种）丙酮酸的可逆羧基化，形成草酰乙酸。

丙酮酸

草酰乙酸

Pyruvate carboxylase (PC) is an enzyme of the ligase class that catalyzes the (depending on the species) irreversible carboxylation of pyruvate to form oxaloacetate (OAA).

Pyruvic acid

Oxaloacetic acid

It is an important anaplerotic reaction that creates oxaloacetate from pyruvate. The enzyme is a mitochondrial protein containing a biotin prosthetic group, requiring magnesium or manganese and acetyl CoA.