The expression product of ScFv was purified through Ni-NTA agarose metal affinity resin column and renatured. 经金属螯合亲和层析柱(Ni-NTA agarose)纯化和凝胶柱上在位复性后,ELISA结果证实其具有良好的结合β3抗原活性。
When the denatured reduced egg white lysozymes were renatured by dilution method, an observable amount of aggregate precipitate could be immediately formed. 当用复性液稀释复性还原服变性蛋白溶菌酶时,会迅速产生可观量的沉淀。